Temperature-sensitive mutant strains for isolation of additional mutants of a given site
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منابع مشابه
Isolation of temperature-sensitive mutants of L-cells.
Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine.
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Measles virus differs from other members of paramyxoviruses in its prolonged growth cycle1), its degree of involvement of the cell nucleus2), its lack of neuraminidase3) and the sedimentability of its RNA4). We have undertaken a study of mutants of this virus in the hope that the study might circumvent some of the problems encountered with temperaturesensitive (ts) mutants of other paramyxoviru...
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Three separate murine sarcoma virus nonproducer cell lines have been isolated which are temperature sensitive for the maintenance of transformation. In each case, a viral rather than a cellular genetic mutation is the reason for the temperature-sensitive effect. Superinfection of one of the mutants with murine leukemia virus overcomes the temperature-sensitive change in the transformed state.
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One hundred and twenty-one sensitive (ts) sex-linked lethals were screened by means of X-ray-induced somatic crossing over to determine if any were ts cell-lethal mutants. Cell-lethal mutations were identified by their ability to block the development of homozygous clones when raised under restrictive conditions (29degrees). Twenty-two ts cell-lethal mutants were isolated and categorized into t...
متن کاملIsolation and characterization of Sendai virus temperature-sensitive mutants.
Ten temperature-sensitive mutants of Sendai virus, a paramyxovirus, were isolated and partially characterized. The mutants replicated in chicken embryo lung cells at 30 C, but not at 38 C; wild-type virus grew equally well at both temperatures. Complementation tests divided the mutants into seven groups. Six groups synthesized neither infectious virus nor RNA when incubated at 38 C from the beg...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1966
ISSN: 1941-4765
DOI: 10.4148/1941-4765.2033